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本文介绍了胰岛素、Ｃ肽ＲＩＡ检测中的三种室内质控方法，即质控血清监测法，两样本Ｙｏｕｄｅｎ图监测法和标准曲线参数监测法，并对其结果做了初步分析。三种室内质控方法的应用，保证了每一批测定结果的准确可靠、提高了不同批实验结果的可比性。本文还对胰岛素、Ｃ肽ＲＩＡ检测中使用的试剂盒进行了方法学评价。胰岛素、Ｃ肽测定的批内ＣＶ分别为５．４８％和９．９８％；批间ＣＶ分别为１０．５４％和１４．５６％；平均回收率分别为１０２．６％和９８．７％；灵敏度分别为１．２５ｍＵ／Ｌ和０．０２ｎｍｏｌ／Ｌ。测定了１８４名正常人空腹血清胰岛素和Ｃ肽的含量，分别为８．３５±４．４７ｍＵ／Ｌ和０．２５～１．３２ｎｍｏｌ／Ｌ。与文献报告的正常值接近。
In this paper, three methods for quality control of insulin and C-peptide RIA are described, that is, quality control serum monitoring method, two-sample Youden graph monitoring method and standard curve parameter monitoring method, and their results are analyzed preliminarily. The application of three kinds of indoor quality control methods ensures the accuracy and reliability of each batch of measurement results and improves the comparability of different batch experimental results. This article also on insulin, C peptide RIA test kit used in the methodological evaluation. The intra-assay CV of insulin and C-peptide was 5.48% and 9.98%, respectively. The inter-assay CV was 10.54% and 14.56%, respectively. The average recoveries were 102.6% and 98.7% ; Sensitivity were 1.25mU / L and 0.02nmol / L respectively. The fasting serum insulin and C-peptide levels were determined in 184 normal individuals, 8.35 ± 4.47mU / L and 0.25 ~ 1.32nmol / L, respectively. Close to the normal value reported in the literature.