新采收的山葡萄（ＶｉｔｉｓａｍｕｒｅｎｓｉｓＲｕｐｒ．）种子风干后含水１０．９２％，硅胶干燥使其含水量分别降至６．２％和３．５％。经过干燥脱水的种子ＴＴＣ染色百分率同风干种子相比没有差异，均为１００％。种子密封置室温、０～５℃、－１８℃及－１９６℃下保存５年。室温下保存的种子全部丧失生活力，低温下保存的种子均有存活，其中以－１９６℃含水量３．５％的种子出苗率较最高，达７４％。试验证明山葡萄种子含水量降至３．５％不损害种子生活力，并可用低温或超低温保存，应确定为正常型种子。 Freshly harvested Vitis amurensis Rupr. Seeds dried 10.92% air dried and dried to a moisture content of 6.2% and 3.5%, respectively. The percentage of TTC stained dried desiccated seeds was 100% compared to air-dried seeds. Seed seal set at room temperature, 0 ~ 5 ℃, -18 ℃ and -196 ℃ for 5 years. The seeds stored at room temperature all lost their viability, and all the seeds preserved at low temperature survived. Among them, the seed germination rate at the water content of -196 ℃ 3.5% was the highest, reaching 74%. Experiments show that the grape seed water content reduced to 3.5% does not affect the viability of seeds, and can be cryogenic or cryogenic preservation, should be identified as normal seed.