从EDTA处理的重组E.coli中分离纯化重组人SOD包涵体

来源 :中国医药工业杂志 | 被引量 : 0次 | 上传用户:zhp95869213
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
以金属离子螯合剂乙二胺四乙酸(EDTA)代替超声法使重组大肠杆菌菌体释放,并提取重组人超氧化物歧化酶包涵体,纯度为65%。采用透析法复性包涵体,经凝胶色谱纯化后,目的蛋白纯度约为90%,酶比活力为5400u/(mg蛋白)。
其他文献
在市教研室的“三人行”展示课活动中,有新教师执教《老王》一课,听后感触不少,回来感觉意犹未尽,上网一浏览,发现青春课堂代表人物——特级教师王君也曾上过该课。网上还有她的课堂实录,于是饕餮了一顿精神大餐。她在课尾结束时说:“孩子们,杨绛是幸运的人吗?她也是一个活命的人啊。为什么她也活命,她却说自己是幸运的?为什么她还愧怍?你们现在也许还不会太懂。但有一天,你们会懂……”看来如何理解“幸运”,如何理解
BACKGROUND Ulcerative colitis(UC)is a chronic disease characterized by inflammation of intestinal epithelium,primarily of the colon.An increasing prevalence of
AIM To determine if end-stage renal disease(ESRD)is a risk factor for post endoscopic retrograde cholangiopancreatography(ERCP)adverse events(AEs).METHODS We pe
AIM To investigate the association between smoking habits and surgical outcomes in hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC)(B-HCC)and hepati
Ulcerative colitis(UC),a chronic,relapsing,remitting disease of the colon and rectum,is characterized by inflammatory ulceration of the mucosa.Current UC therap
AIM To investigate the accuracy of fungal dysbiosis in mucosa and stool for predicting the diagnosis of Crohn’s disease(CD).METHODS Children were prospectively
灯迎着它眼前一片灿烂的光明转个面身后一串长长的黑影这首小涛,短短四句,貌不惊人,平平淡淡,寻寻常常,但要慢慢咀嚼,细细涵润,却趣味横生,见仁见智,侧峰横岭。花筒万变,不及其趣,潭水干
AIM To identify and predict the competing endogenous RNA(ceRNA)networks in colorectal cancer(CRC)by bioinformatics analysis.METHODS In the present study,we obta
AIM To screen clinically relevant micro RNAs (mi RNAs) silenced by DNA methylation in human hepatocellular carcinoma(HCC).METHODS Knockdown of DNA methyltransfe
AIM To investigate the surgical therapies for gastric cancer(GC) patients of age 85 or older in a multicenter survey.METHODS Therapeutic opportunities for elder