论文部分内容阅读
革兰阴性杆菌脓毒症的临床表现是由细菌毒素、宿主介质和细菌胞壁成份释放入血引起,其中脂多糖中的脂A组份较为重要。文内介绍了清除循环中细菌毒素的实验研究方法。活体实验选用78只成年Wister鼠,经两侧颈内静脉输入对庆大霉素敏感的大肠杆菌4.6～6.2×10~8菌落形成单位(CFU)/ml,此剂量约为试验动脉致死量的80％。其中24只以股动、静脉与直接灌流系统(DHP)连接行体外灌流。DHP中装有玻璃珠或1克多粘菌素聚苯
The clinical manifestations of Gram-negative bacilli sepsis are caused by the release of bacterial toxins, host mediators and bacterial cell wall components into the bloodstream, of which lipid A component is more important. The article describes the removal of circulating bacterial toxins experimental research methods. In vivo experiments, 78 adult Wister rats were selected and gentamicin-sensitive colony-forming units (CFU) / ml were injected via both jugular veins on both sides. The dose was about the test arterial lethal dose 80%. Twenty-four of them were externally perfused with femoral artery, vein and direct perfusion system (DHP). DHP is filled with glass beads or 1 gram polymyxin polybenzene