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水稻的倒伏问题一直是制约其产量的重要因素之一。不断发掘、利用水稻抗倒伏性状相关QTL,选育出抗倒伏品种,从而提高水稻收获指数,是新时期水稻理想株型和高产育种相结合的重要育种方向。本研究利用Vary Lava 1312和华恢1 462构建的F2:3群体,基于全基因组构建遗传连锁图谱,对水稻基部茎秆直径(BCM)、伸长第一节茎秆直径(FCM)、伸长第二节茎秆直径(SCM)和株高(PH)进行QTL(quantitative trait locus)分析。2013年和2014年两年表型数据共检测到19个控制茎宽的QTLs和4个控制株高的QTLs。其中,位于第1染色体和第9染色体上的5个茎宽QTLs(q BCM1,q BCM9,q FCM1,q FCM9.1和q SCM1)和2个株高QTLs(q PH1.1和q PH9)在两年中均被检测到。位于第1染色体上RM4011-RMYP41231区间内的QTL在两年重复中均具有较好的稳定性。此区间的QTLs对各节茎秆直径的表型变异解释率为2.69%~16.22%,这些稳定检测到的QTLs对于利用分子标记辅助选择改良水稻抗倒伏提供了重要的基因资源。
The lodging problem of rice has always been one of the important factors restricting its output. It is an important breeding direction for the combination of ideal plant type and high-yielding rice in the new era to continuously explore and utilize the QTLs related to lodging resistance of rice to breed lodging-resistant varieties and improve the harvest index of rice. In this study, based on the genome-wide genetic linkage map, the F2: 3 populations of Vary Lava 1312 and Huahui 1 462 were used to analyze the relationship between the stem diameter (BCM) and the stem diameter (FCM) The second section of stem diameter (SCM) and plant height (PH) QTL (quantitative trait locus) analysis. A total of 19 QTLs controlling stem width and 4 QTLs controlling plant height were detected in the two years 2013 and 2014 phenotypic data. Among them, five stem QTLs (q BCM1, q BCM9, q FCM1, q FCM9.1 and q SCM1) and two QTLs (q PH1.1 and q PH9) located on chromosome 1 and chromosome 9, In two years were detected. The QTL located on the RM4011-RMYP41231 interval on chromosome 1 showed good stability in two years of replication. The QTLs in this interval explained the phenotypic variation of the stalk diameters of all the segments from 2.69% to 16.22%. These QTLs, which were detected stably, provided an important genetic resource for improving rice lodging resistance by using molecular marker-assisted selection.