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1979年我们以冬小麦(有芒红7号×洛夫林10号)5190×京双2号F_1代花药为材料。按常规的花培无菌操作程序进行接种和培养。培养基为N_6+LH500毫克/毫升+2,4-D2毫克/毫升+KT1毫克/毫升+蔗糖8％+琼脂0.8％、N_6+LH500毫克/毫升+2,4-D2毫克/毫升+蔗糖8％+琼脂0.8％和C_1+V_B10.4毫克/毫升+肌醇100毫克/毫升+生物素0.5毫克/毫升+2,4-D3毫克/毫升+KT0.3毫克/
In 1979 we used winter wheat (with Mangoshen 7 × Lovrin 10) 5190 × Jingshuang 2 F_1 anthers as material. Inoculation and culture are carried out according to the conventional flower culture asepsis procedure. Medium: N_6 + LH500 mg / ml + 2,4-D2 mg / ml + KT1 mg / ml + sucrose 8% + agar 0.8%, N_6 + LH500 mg / ml + 2,4-D2 mg / ml + sucrose 8 % + Agar 0.8% and C_1 + V_B10.4 mg / ml + inositol 100 mg / ml + biotin 0.5 mg / ml + 2,4-D3 mg / ml + KT0.3 mg /