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在室内研究了分离自小猿叶甲的一株球孢白僵菌(SCAU-BB 01D)对小猿叶甲的致病力。结果表明,该菌株能感染小猿叶甲的成虫和各龄幼虫,但对不同虫期的致病力存在差异。在105~108孢子/m l的浓度范围内,随着处理浓度的升高,各虫期小猿叶甲的感病死亡率增加,在最高浓度1×108孢子/m l,处理后成虫第14天及1~3龄幼虫第10天的累计死亡率分别为84.7%、94.0%、96.0%和81.0%。用TDM模型对成虫和各龄幼虫的致病力数据进行模拟,所建模型均顺利通过Hosm er-L em eshow拟合异质性检验,表明模型拟合良好,并由模型估计出了该菌株对小猿叶甲各虫期的致死剂量与致死时间。在处理后第10天,成虫和1~3龄幼虫的致死中浓度(LC50)分别为2.68×107、1.07×106、1.63×105孢子/m l和8.31×106孢子/m l,而第14天成虫的LC50为2.38×106孢子/m l。随着浓度的增加,各虫期所需的感病死亡时间缩短,在最高浓度1×108孢子/m l,球孢白僵菌对小猿叶甲成虫及1~3龄幼虫的致死中时(LT50)分别为9.28、4.29、4.40d和5.06 d。综合分析白僵菌对各虫期的致死剂量及致死时间可以看出,不同虫期的小猿叶甲对球孢白僵菌敏感性不同。结果表明该菌株在小猿叶甲生物防治中具有一定的潜力。
The virulence of a strain of Beauveria bassiana (SCAU-BB 01D) isolated from Pedicularis arifolius to Pedigree was studied indoors. The results showed that the strain could infect the adults and the larvae of the leafhopper, but different pathogenicity in different stages. In the range of 105-108 spores / ml, the susceptible mortality of P. chinensis increased with the increase of the treatment concentration at the highest concentration of 1 × 108 spores / ml and the 14th day after the treatment And the cumulative mortality of the 1st to 3rd instar larvae on the 10th day were 84.7%, 94.0%, 96.0% and 81.0%, respectively. Pathogenicity data of adult and juvenile larvae were simulated with TDM model. The models were successfully fitted by Hosmer-Lemshow heterogeneity test, which showed that the model fitted well and the model was used to estimate the virulence of the strain The lethal dose and lethal time to each stage of. On day 10 after treatment, the lethal concentrations (LC50) of adult and 1st to 3rd instar larvae were 2.68 × 107, 1.07 × 106, 1.63 × 105 spores / ml and 8.31 × 106 spores / ml, respectively. On the 14th day adult The LC50 was 2.38 x 106 spores / ml. With the increase of concentration, the required death time of infection in each stage was shortened. At the highest concentration of 1 × 108 spores / ml, the mortality of Beauveria bassiana and 1 ~ 3 instar larvae LT50) were 9.28, 4.29, 4.40 d and 5.06 d, respectively. Based on a comprehensive analysis of the lethal dose and lethal time of Beauveria bassiana to each stage, we can see the different sensitivities to different stages of Beauveria bassiana to Beauveria bassiana. The results showed that the strain has certain potential in the biological control of the minnow.