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神舟三号飞船搭载带核径迹辐射探测器的水稻种子装置,回收后应用随机扩增多态性DNA(randomamplified polymorphic DNA,RAPD)技术,分析了201粒升空种子长出植株的基因组多态性。在检测的189个基因座位范围内,30.2%的植株中发现与地面对照不同的扩增带,单株的多态性座位数为1￣25。特异扩增带的测序及单核苷酸多态性(single-nucleotide polymorphism,SNP)分析进一步证明了空间搭载水稻种子确实可导致当代植株基因组发生变异。同一技术分析个别种子连续世代的基因组多态性,结果显示,当代的部分多态性可遗传至后代。7粒受空间高原子序数、高能粒子轰击的种子,在当代植株均显示不同程度的基因组多态性,从胚受粒子击中的3粒种子后代中,筛选出农艺性状明显变异的突变株系,初步暗示了空间高能重离子辐射对诱导基因组的多态性,乃至遗传性表型变异的有效性。
The Shenzhou 3 spacecraft was equipped with a rice seed device with a nuclear track radiation detector. After being recovered, random amplified polymorphic DNA (RAPD) was used to analyze the genomic polymorphism of 201 seeds Sex. In the range of 189 loci detected, 30.2% of the plants found bands different from the ground control, and the number of loci per plant was 1 ~ 25. Sequencing of SNPs and single-nucleotide polymorphism (SNP) analysis further demonstrated that space-borne rice seeds can indeed lead to genome-wide variation in contemporary plants. The same technique was used to analyze the genomic polymorphisms of successive generations of individual seeds. The results show that some of the contemporary polymorphisms can be inherited to offspring. Seven seeds, which were bombarded by high energy atomic number and high energy particles, showed different degrees of genomic polymorphism in contemporary plants. Among the three seed progenies hit by seeds, the mutant lines with obvious variation of agronomic traits , Initially hinted that the space high-energy heavy ion radiation on the induced genome polymorphism, and even the effectiveness of genetic phenotypic variation.