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目的:探讨miR-223的表达与胃癌细胞的增殖能力的关系。方法:采用实时定量PCR检测人胃癌组织及细胞系中miR-223的表达。采用四甲基偶氮唑蓝(MTT)法和克隆形成实验测定转染miR-223 inhibitors前后的胃癌细胞增殖的变化。结果:相对于正常胃粘膜组织及细胞,在胃癌组织和细胞中miR-223的表达水平出现了显著的上调,MGC-803细胞中转染miR-223 inhibitors能显著抑制miR-223的表达水平。抑制miR-223能降低胃癌细胞的增殖能力。结论:miR-223对胃癌细胞增殖的调控至关重要。miR-223的表达降低能显著抑制胃癌细胞的增殖能力。
Objective: To investigate the relationship between the expression of miR-223 and the proliferation of gastric cancer cells. Methods: Real-time quantitative PCR was used to detect the expression of miR-223 in human gastric cancer tissues and cell lines. The proliferation of gastric cancer cells before and after transfected with miR-223 inhibitors was measured by MTT assay and clonogenic assay. Results: Compared with normal gastric mucosa tissues and cells, miR-223 expression was significantly up-regulated in gastric cancer tissues and cells. MiR-223 inhibitors transfected into MGC-803 cells could significantly inhibit the expression of miR-223. Inhibition of miR-223 can reduce the proliferation of gastric cancer cells. Conclusion: The regulation of miR-223 on the proliferation of gastric cancer cells is crucial. The decrease of miR-223 expression can significantly inhibit the proliferation of gastric cancer cells.