论文部分内容阅读
目的探讨雷公藤内酯醇(Tri)对实验性自身免疫性脑脊髓炎(EAE)外周血及中枢神经系统中CD4+T淋巴细胞的影响,为Tri临床应用提供实验依据。方法建立EAE大鼠模型,给予Tri预防性治疗,流式细胞术检测大鼠外周血的CD4~+T淋巴细胞变化,免疫组化观察中枢神经系统中CD4+T淋巴细胞的浸润情况。结果与生理盐水模型组比较,Tri治疗组的发病率显著降低,中枢神经系统中CD4~++ T淋巴细胞的浸润显著减少,而外周血CD4~+T淋巴细胞的量变化不大。结论Tri可能通过抑制CD4+T淋巴细胞在中枢神经系统的浸润,而阻止EAE的发病,提示Tri在治疗人类多发性硬化方面有一定应用前景。
Objective To investigate the effects of triptolide (Tri) on CD4+ T lymphocytes in peripheral blood and central nervous system of experimental autoimmune encephalomyelitis (EAE), providing experimental basis for Tri clinical application. Methods A rat model of EAE was established and Tri preventive treatment was performed. Flow cytometry was used to detect the changes of CD4~+ T lymphocytes in peripheral blood of rats. Immunohistochemistry was used to observe the infiltration of CD4+ T lymphocytes in the central nervous system. Results Compared with the saline model group, the incidence of the Tri treatment group was significantly reduced, CD4~++ T lymphocyte infiltration in the central nervous system was significantly reduced, and the amount of CD4~+ T lymphocytes in the peripheral blood was not changed significantly. Conclusion Tri may prevent the onset of EAE by inhibiting the infiltration of CD4+ T lymphocytes in the central nervous system, suggesting that Tri has a certain application prospect in the treatment of multiple sclerosis in humans.