VP1为感染并裂解霍乱弧菌的噬菌体,全基因组为环状双链DNA。通过测定该基因组序列,预测出15个可能的启动子区,利用报告基因质粒转化及全噬菌体共感染的策略分析了这些推测启动子在霍乱弧菌中的活性,将预测的启动子区分别克隆到启动子探测lacZ融合质粒载体pRS1274,在转化于大肠埃希氏菌受体菌株JM109中时,所有克隆子均呈现兰斑。同时将质粒电击到缺失了lacZ基因的霍乱弧菌菌株7743△Z,然后用噬菌体VP1感染转化菌株。在转化成功的13个含预测启动子片段的重组质粒中,通过检测β_半乳糖苷酶活性表达随感染后时间的变化,提示P17为早期启动子,P2、P3、P9等为中期启动子,P18为晚期启动子。 VP1 is a bacteriophage that infects and cleaves Vibrio cholerae, and the whole genome is circular double-stranded DNA. Fifteen possible promoter regions were predicted by sequencing the genomic sequences. The putative promoters were analyzed for their activity in V. cholerae using reporter gene plasmid transformation and whole phage co-infection strategies. The predicted promoter regions were cloned separately To the promoter detection lacZ fusion plasmid vector pRS1274, when transformed into Escherichia coli receptor strain JM109, all the clones showed blue spot. At the same time, the plasmid was shocked to Vibrio cholerae strain 7743ΔZ lacking the lacZ gene, and the transformed strain was then infected with phage VP1. The results showed that P17 was an early promoter and P2, P3, P9 and so on were mid-stage promoters in the recombinant plasmids containing 13 predicted promoters which had been transformed successfully by detecting the change of β-galactosidase activity with the time after infection , P18 is a late promoter.