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目的 探讨P16基因缺失与非小细胞肺癌病理类型、临床分期的关系。方法 采用聚合酶链反应 (PCR)技术 ,检测 3 1例非小细胞肺癌及相应匹配癌旁正常组织中P16基因外显子 2的缺失情况。结果 3 1例非小细胞肺癌组织中 ,7例P16基因纯合性缺失 ,缺失频率为 2 2 .6 %。其中鳞癌 6例 ,腺癌 1例。 7例缺失均为Ⅲ、Ⅳ期临床病例。结论 P16基因缺失可能在非小细胞肺癌的发生、发展中起作用 ,并与病理类型、临床分期有关。
Objective To investigate the relationship between deletion of P16 gene and pathological type and clinical stage of non-small cell lung cancer. Methods Polymerase chain reaction (PCR) was used to detect the deletion of exon 2 of P16 gene in 31 cases of non-small cell lung cancer and corresponding matched normal tissues. Results Of the 31 non-small cell lung cancer tissues, homozygous deletion of P16 gene was found in 7 cases, and the frequency of deletion was 22.6%. There were 6 cases of squamous cell carcinoma and 1 case of adenocarcinoma. The 7 cases were all clinical stages III and IV. Conclusion The deletion of P16 gene may play a role in the occurrence and development of non-small cell lung cancer, and it is related to pathological types and clinical stages.