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NPCEDRG基因是采用基因定位候选克隆策略获得的1个鼻咽癌候选抑瘤基因.NPCEDRG在鼻咽癌细胞和组织中表达下调,重新恢复NPCEDRG基因在CNE2细胞系的表达,可部分逆转CNE2的恶性表型.本研究对CNE2细胞所表达的NPCEDRG基因mRNA剪接变异体克隆、鉴定,发现NPCEDRG基因至少有7个转录起始位点,其中NM_032316的TSS位于ATG上游-85 nt处,AF538150和AK094248的TSS位于-25 nt处;AF538150不存在第2外显子中6核苷酸序列(3’-TTGCAG-3’)的缺失,其CDs为516 bp,编码1种由171个氨基酸组成的蛋白质(而非GenBank中公布的CDs为510 bp,1种由169个氨基酸组成的蛋白质).本研究成功克隆得到1种新的NPCEDRG基因的mRNA剪接变异体V2,其TSS位于-23 nt处,其CDs为297 bp,编码1种由98个氨基酸组成的蛋白质.
NPCEDRG gene is a nasopharyngeal candidate tumor suppressor gene obtained by gene cloning candidate cloning strategy.NPCEDRG is downregulated in NPC cells and tissues and regained the expression of NPCEDRG gene in CNE2 cell line and partially reversed the malignancy of CNE2 In this study, CNE2 cells expressed NPCEDRG gene mRNA splicing variant cloning, identification and found NPCEDRG gene at least seven transcriptional start sites, including TS_ TS_ NM_032316 is located at -85 nt upstream ATG, AF538150 and AK094248 TSS is located at -25 nt; AF538150 does not exist the deletion of the 6 nucleotide sequence (3’-TTGCAG-3 ’) in exon 2, and its CDs is 516 bp, encoding a protein consisting of 171 amino acids While non-GenBank published CDs of 510 bp and a protein consisting of 169 amino acids.) In this study, we successfully cloned a new mRNA alternative splicing variant of NPCEDRG V2, whose TSS is located at -23 nt, and its CDs Is 297 bp encoding a protein consisting of 98 amino acids.