论文部分内容阅读
目的 观察亚硒酸钠 (sodium selenite,Na2 Se O3)对微波致体外培养的视网膜神经节细胞损伤的防护作用。 方法 体外培养视网膜神经节细胞 ,按加入培养液 Na2 Se O3浓度分为 4组 ,其中 1组为对照组 ,30 m W/ cm2 微波辐射 1h,测定细胞内的脂质过氧化指标及硒浓度 ,末端脱氧核甘酸转移酶介导的生物素化脱氧尿甘三磷酸末端标记法 (Td T- mediated d UTP nick end labeling,TUNEL )检测细胞凋亡。 结果 1× 10 - 7mol/ L Na2 Se O3即可使细胞内超氧化物歧化酶 (superoxide dismutase,SOD)、谷胱甘肽过氧化物酶 (glutatione peroxidase,GSH- Px)活性较未加硒组明显升高 (P<0 .0 5 ) ,丙二醛 (malondialdehyde,MDA)含量显著下降 (P<0 .0 5 ) ,凋亡细胞数量减少 ,1× 10 - 6 mol/ L组和 1× 10 - 5 mol/ L组在抗氧化能力方面优于1× 10 - 7mol/ L 组 ,但这两组间无显著差异。 结论 Na2 Se O3对微波致视网膜神经节细胞的损伤具有一定的保护作用。
Objective To observe the protective effect of sodium selenite (Na2 Se O3) on retinal ganglion cell injury induced by microwave in vitro. METHODS: Retinal ganglion cells were cultured in vitro and divided into 4 groups according to the concentration of Na2Se O3 added into the culture medium. One group was the control group, and the lipid peroxidation index and selenium concentration in the cells were determined by microwave irradiation at 30 mW / cm2 for 1 h. Apoptosis was detected by TdT-mediated dUTP nick end labeling (TUNEL). Results The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH - Px) in the cells treated with 1 × 10 -7 mol / L Na 2 Se 3 O were higher than those without selenium (P <0.05), the content of malondialdehyde (MDA) decreased significantly (P <0.05), the number of apoptotic cells decreased, the number of apoptotic cells in 1 × 10-6 mol / L group and 1 × The 10 - 5 mol / L group was superior to the 1 × 10 - 7 mol / L group in antioxidant capacity, but there was no significant difference between the two groups. Conclusion Na2 Se O3 has a protective effect on the damage of retinal ganglion cells induced by microwave.