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SLE是一种病因未明的自身免疫性疾病。现已发现多个HLA等位基因与SLE易感性相关,但原始相关基因仍未确定。TNFB基因位HLA-B和C4A基因之间,其编码的TNF-B是一种在细胞因子网络免疫调节中起重要作用的细胞因子。故此作者认为TNFB基因为SLE易感基因的候选基因。作者随机选择了173例德国SLE病人和192例无关健康者,这些调查对象均已进行过HLA-I血清法定型和HLA-Ⅱ的PCR/SSC及C4的RFLP定型,用引物TNF-BL和R扩增基TNFB基因,并用Nco I消化酶消化分析其RFCP的分布并进行比
SLE is an unknown autoimmune disease. It has been found that multiple HLA alleles are associated with susceptibility to SLE, but the original related genes have not been identified. The TNFB gene between the HLA-B and C4A genes encodes TNF-B, a cytokine that plays an important role in the immunoregulation of cytokine networks. Therefore, the authors believe that the TNFB gene is a candidate gene for SLE susceptibility genes. The authors randomized 173 German patients with SLE and 192 unrelated healthy individuals. All of these subjects had undergone HLA-I serotyping and HLA-II PCR / SSC and C4 RFLP typing. The primers TNF-BL and R The TNFB gene was amplified and analyzed for its RFCP distribution with Nco I digestion and digestion