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以三聚氰胺(MAM)为模板分子,甲基丙烯酸(MAA)为功能单体,二甲基丙烯酸乙二醇酯(EGDMA)为交联剂,原位聚合法制备了对MAM有很强选择性识别能力的分子印迹整体柱。采用脉冲洗脱法快速筛选MAM的洗脱剂,通过前沿色谱法测定了整体柱对MAM的结合容量。实验表明,所制备的印迹整体柱对MAM有极强亲和作用力,即使在强极性流动相中(甲醇或甲醇-水(V/V=80/20)),MAM在印迹柱上也有强保留,不被洗脱。实验测得结合位点数(Lt)和解离常数(Kd)分别为:印迹柱Lt=2.28×103μmol/g,Kd=2.45×10-5mol/L;空白柱Lt=366μmol/g,Kd=23.7mo/L。该印迹整体柱有望作为固相萃取柱,在线或离线选择性富集样品中的MAM。
Using MAM as template molecule, methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinking agent, in-situ polymerization method was used to prepare MAM with strong selective recognition Ability of molecular imprinting monoliths. The eluent of MAM was rapidly screened by pulse elution and the binding capacity of the monolith to MAM was determined by frontier chromatography. Experiments show that the prepared monolithic imprinted column has a strong affinity for MAM even in highly polar mobile phases (methanol or methanol-water (V / V = 80/20)) and MAM on imprinting columns Strongly reserved, not to be eluted. The experimental results showed that Lt = 2.28 × 103μmol / g and Kd = 2.45 × 10-5mol / L respectively; Lt = 366μmol / g, Kd = 23.7mo / L. The imprinted monolith is expected to be used as a solid-phase extraction column for the selective enrichment of MAM in a sample online or offline.