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目的目前的硫化氢(H2S)研究仍缺乏理想的供体。本研究旨在利用介孔二氧化硅纳米粒子(MSN)合成一种新型的控释H2S供体,并评价其在体外实验中释放H2S的特点。方法溶胶-凝胶法制备均一大小的MSN,将二烯丙基三硫化物(DATS)负载至MSN孔道中,合成控释H2S系统(DATS-MSN)。分析其表征、释放H2S的特点、细胞毒性和细胞摄取能力。结果 DATS-MSN可在体外缓慢、持续、可调节的释放H2S。其可被心肌细胞成功摄取,在常规应用的浓度范围内未见明显细胞毒性。结论 DATS-MSN可在体外环境中缓慢而可控的释放H2S,并具备良好的体外安全性,是H2S体外研究的良好工具。
Objective The current hydrogen sulfide (H2S) research still lacks an ideal donor. The purpose of this study was to synthesize a new controlled-release H2S donor using mesoporous silica nanoparticles (MSN) and to evaluate its release of H2S in vitro. Methods A uniform size MSN was prepared by sol-gel method. Diallyl trisulfide (DATS) was loaded into the MSN channel to synthesize a controlled-release H2S system (DATS-MSN). Analysis of its characterization, the characteristics of release of H2S, cytotoxicity and cellular uptake capacity. Results DATS-MSN can release H2S slowly and continuously in vitro. It can be successfully taken up by cardiomyocytes, with no apparent cytotoxicity at routinely used concentrations. Conclusion DATS-MSN can slowly and controllably release H2S in vitro and has good in vitro safety. It is a good tool for H2S in vitro study.