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目的 观察阿托伐他汀对THP-l巨噬细胞源性泡沫细胞载脂蛋白M(ApoM)表达的影响.方法 培养THP-1单核细胞,序贯加入佛波酯(PMA)和氧化低密度脂蛋白(ox-LDL)进行诱导,使其分化为泡沫细胞,RT-qPCR和油红O染色鉴定.泡沫细胞模型建立后,加入阿托伐他汀处理,RT-qPCR和Western blot检测ApoM的mRNA和蛋白的表达变化,使用人pre-β-HDL酶联免疫试剂盒检测细胞培养基中pre-β-HDL水平.结果 阿托伐他汀处理后的泡沫细胞较对照组和DMSO组ApoM mRNA和蛋白表达均有升高(P<0.05).与对照组和DMSO组细胞上清中pre-β-HDL表达量相比较,阿托伐他汀组上清中pre-β-HDL表达量有明显升高(P<0.05).结论 阿托伐他汀能上调泡沫细胞ApoM的表达,继而刺激pre-B-HDL的合成和分泌,促进胆固醇从泡沫细胞内流出.ApoM可能是他汀类药物的重要降脂机制之一.“,”Objective To investigate the effect of atorvastatin on the expression of apolipoproteinM (ApoM) in foam cells.Methods The THP1 cells were cultured and incubated with phorbool-l-myristate-13-acetate (PMA) and oxidized low density lipoprotein (ox-LDL) to establish foam cell model.RT-qPCR and oil red O staining were used to identify foam cells.RT-qPCR and Western blot were performed to analyze the mRNA and protein expression levels of ApoM in atorvastatin treated foam cells.The concentration of pre-β-HDL in culture media was measured by human pre-β-HDL enzyme-linked immunosorbent assaykit.Results Compared with the control group and the DMSO group,Atorvastatincan statistically increase the expression of ApoM(P<0.05).The level of pre-β-HDLin culture media of atorvastatin treated foam cells is significantly higher than the other two groups.Conclusions Hypolipidemic effect of atorvastatin may be attributed to up-regulating of ApoM expression.