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目的探讨红霉素对支气管哮喘(简称哮喘)大鼠模型肺组织Clara细胞数量和Clara细胞分泌蛋白-16(CC16)表达的影响。方法用卵白蛋白(OVA)致敏、激发建立大鼠哮喘模型,随机分为正常对照组、哮喘组、红霉素组、地塞米松组。收集肺泡灌洗液(BALF)进行细胞计数及分类,光镜下观察肺组织病理学变化;ELISA方法检测BALF中CC16含量,用免疫组化的方法检测肺组织中Clara细胞表达变化。结果与正常对照组相比,哮喘组大鼠支气管及血管周围、肺间质内有嗜酸性粒细胞(EOS)及其他炎性细胞浸润,黏液腺增生,黏膜皱折增多,黏膜上皮细胞脱落,可见气道黏液栓,而红霉素组和地塞米松组上述现象明显减轻。肺组织Clara细胞计数显示,哮喘组大鼠终末及呼吸性细支气管上皮Clara细胞数明显少于正常对照组,而红霉素组和地塞米松组较哮喘组均有所增加(P<0.05)。哮喘组EOS百分比(EOS%)显著高于对照组(P<0.01),红霉素组和地塞米松组的EOS%均低于哮喘组(P<0.01)。BALF中CC16含量,哮喘组明显低于正常对照组(P<0.01),而红霉素组和地塞米松组均高于哮喘组(P<0.05)。结论哮喘时肺组织中Clara细胞及CC16的表达减少,红霉素可通过上调肺组织中Clara细胞及CC16的表达来发挥抗炎作用。
Objective To investigate the effect of erythromycin on the number of Clara cells and the expression of Cla16 cell secretory protein-16 (CC16) in the bronchial asthma rat model. Methods The ovalbumin (OVA) sensitized mice were used to establish the model of asthma in rats. The rats were randomly divided into normal control group, asthma group, erythromycin group and dexamethasone group. The bronchoalveolar lavage fluid (BALF) was collected for cell counting and classification. The pathological changes of lung tissue were observed under light microscope. The CC16 content in BALF was detected by ELISA. The expression of Clara in lung tissue was detected by immunohistochemistry. Results Compared with the normal control group, the eosinophils (EOS) and other inflammatory cell infiltration in the bronchi, perivascular and pulmonary interstitium were found in the bronchial asthma group. The number of mucous gland hyperplasia, mucosal folds increased, the mucosal epithelial cells shed, Visible airway mucus plug, while the erythromycin group and dexamethasone group significantly reduced the above phenomenon. The number of Clara cells in the lung tissue showed that the number of Clara cells in the terminal and respiratory bronchial epithelium of the asthma group was significantly less than that of the normal control group, while the erythromycin group and the dexamethasone group were significantly increased (P <0.05 ). EOS% in asthma group was significantly higher than that in control group (P <0.01). EOS% in erythromycin group and dexamethasone group were lower than those in asthma group (P <0.01). The content of CC16 in BALF was significantly lower in asthma group than in normal control group (P <0.01), while erythromycin group and dexamethasone group were higher than those in asthma group (P <0.05). Conclusions The expression of Clara cells and CC16 in lung tissue is decreased during asthma. Erythromycin can exert anti-inflammatory effects by up-regulating the expression of Clara cells and CC16 in lung tissue.