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目的对山豆根多糖进行硫酸化修饰并对其抗HBV活性进行测定。方法提取山豆根多糖,采用氯磺酸-吡啶法对山豆根多糖进行硫酸化修饰,产物经红外光谱鉴定。用MTT法测定该修饰物的抗HBV活性。结果通过山豆根多糖硫酸化前后的红外图谱分析,表明硫酸基与多糖已结合成酯,并且有抗HBV的作用。结论山豆根多糖硫酸化修饰能提高对乙型肝炎病毒细胞转染的2215细胞培养液中HBeAg、HBsAg的抑制作用。
Objective To purify the polysaccharides from Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma Rhei. Methods The polysaccharides of Radix et Rhizoma are extracted and the sulfated polysaccharides of Radix isatidis were modified by chlorosulfonic acid-pyridine method. The products were identified by infrared spectroscopy. The modified anti-HBV activity was measured by MTT assay. Results The analysis of the infrared spectrum before and after the sulfated polysaccharides of Radix et Rhizoma Radix et Rhizoma Radix et Rhizoma indicated that the sulfates and polysaccharides have been combined into esters and have the anti-HBV effect. Conclusion Soybean rhizome polysaccharide sulfation modification can improve the inhibition of HBeAg and HBsAg in 2215 cell culture solution transfected by Hepatitis B virus.