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以东方杉大树基部当年生的幼嫩萌枝为外植体,进行了组织培养技术研究。结果表明,外植体的最佳消毒方法为75%酒精消毒30～60 s后用升汞0.2%浓度消毒10～15 min。采用茎段微型扦插和诱导丛生芽的方法来增殖扩繁,适宜东方杉芽增殖培养基为:MHJ+BA 0.5 mg/L+NAA 0.05 mg/L+L-谷氨酰胺0.5 g/L+活性碳5 g/L+蔗糖2%,茎段腋芽萌发率可达90%。芽伸长生长的培养基为:MHJ+L-谷氨酰胺0.5 g/L+活性碳5 g/L+蔗糖2%,35 d平均增高4.2 cm。试管苗的生根培养基为:1/2 MHJ+L-谷氨酰胺0.5 g/L+活性碳5 g/L+蔗糖1%,最高生根率达75%,以沙为培养基质的效果比琼脂好。生根的组培苗先在沙床上进行炼苗,炼苗成活率达87%,然后移栽到带混合基质的营养钵中育苗,移栽成活率可达90%,育苗1～2个月后移栽大田。
The young shoots from the roots of oriental fir trees were used as explants to study the tissue culture technology. The results showed that the optimal method for disinfection of explants was disinfection with 75% alcohol for 30 ~ 60 s and disinfection with 0.2% mercuric chloride for 10 ~ 15 min. The stems of miniature cutting and induction of tufted bud proliferation and propagation, suitable for the growth medium of Oriental fir shoots: MHJ BA 0.5 mg / L NAA 0.05 mg / L L-glutamine 0.5 g / L + activated carbon 5 g / L + 2% sucrose, stem bud axillary bud germination rate of up to 90%. The medium for elongation growth was: MHJ + L-glutamine 0.5 g / L + activated carbon 5 g / L + sucrose 2%, 35 d average increase of 4.2 cm. The rooting medium for tube seedlings was: 1/2 MHJ + L-glutamine 0.5 g / L + activated carbon 5 g / L + sucrose 1% and the highest rooting rate was 75%. Rooted tissue culture seedlings in the sand bed for the first refining, survival rate of 87%, and then transplanted into a nutrient matrix with mixed substrate seedlings, transplanting survival rate of up to 90%, 1-2 months after seedling Transplanting fields.