论文部分内容阅读
目的探讨皱瘤海鞘不同部位体外抗单纯疱疹病毒Ⅰ型(HSV-Ⅰ)的活性。方法以阿昔洛韦为阳性对照物,利用vero细胞体外增殖模型,通过细胞病变效应法(CPE)和噻唑蓝(MTT)比色法检测细胞活性,测定皱瘤海鞘不同部位抑制HSV-Ⅰ对vero细胞感染的作用。结果 皱瘤海鞘水部位TC50值为7.448 mg/ml,综合药效IC50为0.093 mg/ml,治疗指数TI为80,具有高效低毒性,与阳性对照药物效果相当。皱瘤海鞘氯仿部位和正丁醇部位TC50值为2.299 mg/ml和3.269 mg/ml,综合药效IC50为0.225 mg/ml和0.272 mg/ml,治疗指数TI为10,12。结论 皱瘤海鞘水部位在体外抗HSV-Ⅰ具有良好的活性,氯仿与正丁醇部位活性很弱。
Objective To investigate the anti-herpes simplex virus type 1 (HSV-Ⅰ) activity in different parts of the tumor tissue of the tumor. Methods Acyclovir was used as a positive control. The proliferation of vero cells in vitro was evaluated by cell viability assay (CPE) and thiazolyl blue (MTT) colorimetric assay. HSV-Ⅰ The role of vero cell infection. Results The tumor TC50 value was 7.448 mg / ml and the IC50 was 0.093 mg / ml. The therapeutic index TI was 80, which showed high and low toxicity. The results were as good as that of the positive control. The TC50 value of acrosome and n-butanol were 2.299 mg / ml and 3.269 mg / ml, respectively, and the IC50 values were 0.225 mg / ml and 0.272 mg / ml, respectively. The therapeutic index TI was 10,12. CONCLUSION: The antitumor HSA-Ⅰ has a good activity in the tumor tissue and the activity of chloroform and n-butanol is very weak.