作者研究了三种在真皮底物上重建表皮的实验模型,代替动物用于皮肤药理学研究,在模拟的生理状态下获得了完全正常的表皮,用这种重建表皮评价了维甲酸和激素对靶细胞(角朊细胞)的效应,观察了在这些药物影响下表皮形态和结构的改变。由于这几种重建表皮在牢固的底物上,可升高至气液界面、模拟系统和局部用药以及代谢研究等特点,显示重建表皮在医学和药理学研究方面具有很大潜力。 The authors studied three experimental models for reconstructing the epidermis on a dermal substrate, instead of using the animal for dermal pharmacology, obtaining an entirely normal epidermis under simulated physiological conditions, using this reconstructed epidermis to evaluate the effect of retinoic acid and the hormone pair Target cells (keratinocytes) effects observed under the influence of these drugs to change the morphology and structure of the epidermis. Because of the robustness of these remodeled epidermis to elevated gas-liquid interfaces, simulation systems and topical applications, and metabolic studies, it has been shown that reconstructed epidermals have great potential for medical and pharmacological research.