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以荞麦的成熟胚，幼胚，下胚轴为外植体，在不同激素浓度配比，不同基本培养基质培养基上的组织培养研究表明：以成熟胚为外植体时，对愈伤组织诱导和生长最适培养基是２．４－Ｄ２．０，ＮＡＡ１．０，６－ＢＡ。０．５的Ｂ＿５培养基；以幼胚为外植体时，对愈伤组织的诱导和生长最适合的培养基是２．４－Ｄ２．０，ＮＡＡ１．０，６－ＢＡ０的ＭＳ培养基；以下胚轴为外植体时，Ｂ＿５和ＭＳ两种培养基均不适合愈伤组织的生长。２．４－０对荞麦愈伤组织根的分化有一定的促进作用。
Using the mature embryos, immature embryos and hypocotyls as explants of buckwheat, the tissue culture on medium with different hormone concentrations and on different basic culture mediums showed that when the mature embryos were used as explants, the callus The optimal medium for induction and growth was 2.4-D2.0, NAA1.0,6-BA. 0.5 of B_5 medium; immature embryos as explants, the callus induction and growth of the most suitable medium is 2.4-D2.0, NAA1.0,6-BA0 MS medium When the hypocotyls were explants, neither B_5 nor MS medium was suitable for callus growth. 2.4-0 of buckwheat callus root differentiation have a certain role in promoting.