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通过在小麦花药培养基中添加麦芽糖的试验,发现含有麦芽糖的诱导培养基对小麦花粉愈伤组织的诱导有明显的影响,这种影响不依赖于培养基中麦芽糖的绝对含量,而取决于培养基中蔗糖与麦芽糖的配比(Su/Ma),麦芽糖与9％蔗糖的最适配比浓度为1％～2％,诱导率比对照高126％～138％.诱导、分化培养基中同时附加一定量的麦芽糖,小麦花粉愈伤组织的绿苗分化率明显提高,在本研究的最优组合中,绿苗分化率比对照提高了22.5个百分点。
By adding maltose to the wheat anther culture medium, the inducing medium containing maltose was found to have a significant effect on the induction of wheat pollen callus, independent of the absolute amount of maltose in the medium, depending on the culture The optimum ratio of sucrose to maltose (Su / Ma) was 1% ~ 2%, and the induction rate was 126% ~ 138% higher than that of the control.At the same time, With the addition of a certain amount of maltose, the green plantlet differentiation rate of wheat pollen callus increased obviously. In the optimal combination of this study, the green plantlet differentiation rate increased by 22.5% compared with the control.