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目的:为了比较研究不同品牌基因工程。—:干扰素(IFNa_(2b)的抗肿瘤效应:方法:采用。—干扰素抑制多种肿瘤细胞生长的MTT法。结果:两种不同品牌的10000IU/ml,1000IU/ml和100IU/ml的IFNa_(2b)(干扰能和安达芬)对人脑胶质瘤细胞(SHG—44),人骨肉瘤细胞(HOS—8603),人白血病细胞(HL—60),红白血病细胞(K562)和淋巴瘤细胞(Raji)的生长均有不同程度的抑制作用。其抑制率分别为20％,10％和5％左右。结论:进口和国产的两种不同品牌IFNa_(2b)对上述五种肿瘤细胞株均有较明显的生长抑制效应,而且抑制百分率均相近。
Purpose: To compare different brands of genetic engineering. —:Antitumor effect of interferon (IFNa_(2b): Method: Use. - MTT method for interferon inhibiting growth of various tumor cells. Results: 10000 IU/ml, 1000 IU/ml, and 100 IU/ml for two different brands IFNa_(2b) (interference energy and Andraffin) on human glioma cells (SHG-44), human osteosarcoma cells (HOS-8603), human leukemia cells (HL-60), erythroleukemia cells (K562) and lymph The growth of tumor cells (Raji) was inhibited to varying degrees, and the inhibitory rates were about 20%, 10%, and 5%.Conclusion: Two different brands of imported and domestic IFNα_(2b) were used in the above five tumor cells. All the strains had obvious growth inhibitory effects and the percentages of inhibition were similar.