论文部分内容阅读
目的:研究粉防己碱对培养乳牛基底动脉平滑肌细胞游离钙浓度([Ca~(2+)_i)的影响.方法:利用AR-CM-MIC阳离于测定系统,采用Fura 2-AM为指示剂,测量单个细胞内[Ca~(2+)]_i.结果:粉防己碱10-100μmol/L对培养乳牛基底动脉平滑肌细胞静思[Ca~(2+)]_i无明显影响.在细胞外钙为 1.3 mmo1/L,粉防己碱可浓度依赖性地抑制KCl引起[Ca~(2+)]_i的升高.咖啡因10 mmol/L可诱导一次[Ca~(2+)]_i瞬间快速升高,随后自发回复到静息水平.粉防己碱 10和 30 μmol/L对咖啡因诱导的[Ca~(2+)]_i瞬间升高没有作用,但高浓度(100 μmol几)粉防己碱抑制了[Ca~(2+)]_i瞬间升高.在细胞外钙为1.3 mmol/L,苯肾上腺素10 μmol/L可引起双相[Ca~(2+)]_i变化,包括快速升高相和持续升高相.在细胞外钙为零,苯肾上腺素仅引起[Ca~(2+)]_i的快速升高相.粉防己碱可浓度依赖性地抑制苯肾上腺素引起[Ca~(2+)]_i的持续升高相.但仅高浓度粉防己碱明显抑制了[Ca~(2+)]_i快速升高相.结论:在培养乳牛基底动脉平滑肌细胞,粉防己碱可能通过影响电压依赖性和苯肾上腺素受体介导的钙通道而抑制钙内流.高浓度粉防己碱也可能影响肌浆网钙释放或钙摄取.
Objective: To study the effect of tetrandrine on the free calcium concentration ([Ca 2+ )_i) of cultured calf’s basilar artery smooth muscle cells. METHODS: Using AR-CM-MIC cation detachment measurement system, using Fura 2-AM as an indicator The agent was used to measure [Ca 2+ ]_i in single cells. Results: Tetrandrine 10-100 μmol/L had no significant effect on the meditation of [Ca 2+ ]_i in cultured calf’s basilar artery smooth muscle cells. External calcium is 1.3 mmo1/L. Tetrandrine can inhibit the increase of [Ca 2+ ]_i induced by KCl in a concentration-dependent manner. Caffeine 10 mmol/L can induce primary [Ca 2+ ]_i Instantly rises rapidly and then spontaneously reverts to resting levels. Tetrandrine 10 and 30 μmol/L have no effect on the instantaneous increase of caffeine-induced [Ca 2+ ]_i but high concentrations (100 μmol several) Tetrandrine inhibited the transient increase of [Ca 2+ ]_i. Extracellular calcium was 1.3 mmol/L, and phenylephrine 10 μmol/L could cause biphasic [Ca 2+ ]_i changes. Including the fast-elevation phase and the sustained-elevation phase. When the extracellular calcium is zero, phenylephrine only causes a rapid increase of [Ca 2+ ]_i. Tetrandrine may inhibit phenylephrine in a concentration-dependent manner. Causes the continuous increase of [Ca 2+ ]_i, but only the high concentration of tetrandrine is significantly inhibited The rapid increase of [Ca 2+ ]_i. Conclusion: In the culture of cow basilar artery smooth muscle cells, tetrandrine may inhibit calcium influx by affecting voltage-dependent and phenylephrine receptor-mediated calcium channels. High concentrations of tetrandrine may also affect sarcoplasmic reticulum calcium release or calcium uptake.