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近来为研究抗癌药物对患恶性肿瘤病人体细胞的作用,广泛应用了在骨髓细胞中染色体损伤的细胞遗传学分析。但要分析这些结果相当困难,因为缺乏有关健康人骨髓细胞中染色体自发畸变率的资料。本文对24名年龄在20～60岁的男女两性健康人的2510个骨髓细胞染色体畸变进行细胞遗传学分析。作者采用不经培养获得染色体标本的方法,把骨髓穿刺物放入10毫升含有0.4毫克/毫升秋水仙素及1滴肝素的Hank溶液中,在37℃恒温中培养1个
Recently, in order to study the effect of anticancer drugs on somatic cells of patients with malignant tumors, cytogenetic analysis of chromosomal damage in bone marrow cells has been widely used. However, analysis of these results is quite difficult because of a lack of data on spontaneous chromosomal aberrations in healthy human bone marrow cells. In this study, chromosome aberrations of 2510 bone marrow cells from 24 males and females aged 20-60 years were analyzed by cytogenetic analysis. The authors used non-cultured chromosomal specimens were obtained, the bone marrow aspirate into 10 ml colchicine containing 0.4 mg / ml and 1 drop of Hank’s Hank solution in a constant temperature of 37 ℃ culture 1