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目的:建立酒炙苦参中苦参碱、氧化苦参碱以及氧化槐果碱的含量测定方法.方法:采用HPLC法,色谱柱为大连依利特HYP-ODS柱(250 mm×4.6 mm,5μm),甲醇-磷酸盐缓冲液(12:88)为流动相,流速为1.0 ml·min-1,检测波长为205 nm,柱温为25℃,进样量为20μl.结果:苦参碱、氧化槐果碱、氧化苦参碱分别在0.105~0.420μg、0.760~3.040μg、1.615~6.460μg范围内呈良好线性关系.苦参碱、氧化槐果碱和氧化苦参碱的平均加样回收率分别为99.70%、99.41%、98.60%,RSD分别为1.92%、1.93%、1.79%(n=6).结论:该方法重现性好,准确可靠,专属性强,灵敏度高,可用于酒炙苦参的质量控制.“,”Objective:To develop an HPLC method for determining matrine , oxymatrine and oxysophocarpine in wine-processed Sophora flavescens.Methods:An HPLC method was used with a Dalian Elite HYP-ODS column (250 mm ×4.6 mm, 5 μm).The mobile phase consisted of methanol-phosphate buffer (12:88) with the flow rate of 1.0 ml· min-1 .The detection wavelength was 205 nm, the column temperature was 25℃and the injection volume was 20 μl.Results:The calibration curve was linear within the range of 0.105-0.420 μg for matrine,0.760-3.040μg for oxysophocarpine and 1.615-6.460μg for oxymatrine.The average recovery of ma-trine, oxysophocarpine and oxymatrine was 99.70%(RSD=1.92%), 99.41%( RSD=1.93%) and 98.60%(RSD=1.79%)(n=6), respectively.Conclusion:The method is accurate and reliable with good reproducibility , promising specificity and high sensitivi-ty, which can be used for the quality control of wine-processed Sophora flavescens.