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目的探讨脐血间充质干细胞(MSCs)静脉移植治疗新生鼠缺氧缺血性脑损伤 (HIBD)的可行性。方法将脐血MSCs移植前以DAPI标记,移植鼠在HIBD后第2周经鼠尾静脉注入脐血MSCs,于移植后第1、2和4周随机处死,行脑组织病理形态学观察,并取海马回相同部位的缺血脑组织切片,荧光镜下观察DAPI阳性细胞数。结果脐血MSCs经尾静脉移植后4周,各组鼠死亡率无显著差异,移植组脑病变率显著低于HIBD组,且该组脑组织病变仅偶见轻度,大多接近于正常,未见到重度脑组织病变发生;HIBD后1周左侧大脑缺血水肿区仍见神经细胞肿胀,细胞外间隙增宽,移植治疗1周后左侧脑组织水肿已明显减轻,上述病理组织学变化已不明显,大鼠病灶侧脑内,可见大量的DAPI阳性细胞分布,集中分布于病灶区周围,与宿主脑有机整合,没有明显的界限。结论脐血MSCs移植治疗新生鼠HIBD可以减轻脑水肿和脑损伤,在移植过程中 MSCs可以透过血脑屏障并分布在损伤的脑组织周围,未见植入反应和其他任何副作用。
Objective To investigate the feasibility of intravenous transplantation of cord blood mesenchymal stem cells (MSCs) for neonatal hypoxic-ischemic brain damage (HIBD). Methods Umbilical cord blood MSCs were labeled with DAPI before transplantation. Cord blood MSCs were transplanted into caudal vein of rats 2 weeks after HIBD. The MSCs were randomly sacrificed at 1, 2 and 4 weeks after transplantation. Pathological changes of brain were observed and analyzed. Take the hippocampus back to the same part of the ischemic brain tissue sections, the number of DAPI-positive cells under fluoroscopy. Results After 4 weeks of caudal vein transplantation, the cord blood MSCs had no significant difference in mortality rate between the two groups. The encephalopathy rate in the transplantation group was significantly lower than that in the HIBD group. The pathological changes of the MSCs in this group were only mild, mostly close to normal, See severe brain lesions occurred; HIBD 1 week after the left cerebral edema area still see nerve cell swelling, widening the extracellular space, left a week after transplantation, edema of the brain tissue has been significantly reduced, the histopathological changes It is not obvious that a large number of DAPI positive cells are distributed in the lateral brain of the rat lesion, which are concentrated around the lesion area and have no obvious boundary with the host brain. Conclusion The transplantation of cord blood MSCs can relieve cerebral edema and brain injury in neonatal rats. Transplantation of MSCs can penetrate the blood-brain barrier and distribute around the damaged brain tissue. No implanted reaction and any other side effects are observed.