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已发现细菌感染与胆脂瘤形成有关。角质碎屑的集聚是胆脂瘤生长的决定因素。而耳胆脂瘤又是细菌的一种理想培养基。为探讨细菌感染与胆脂瘤的关系,作者在体外研究了磷壁酸脂(Iipoteichoic acid LTA,一种革兰氏阳性细菌细胞壁成份)对角化细胞增殖和分化的影响。用新生小鼠的皮肤制备基底角化细胞并进行培养。细胞生长阶段,加入不同浓度的 LTA(0～100μg/ml)。然后用~3H 朐腺嘧啶或~3H 亮氨酸标记细胞,并孵育24小时。渗透入角化细胞中的~3H 胸腺嘧啶或~3H 亮氨酸用闪烁记数器测定。并通过检测角化细胞包膜的数及转谷酰胺
It has been found that bacterial infection is associated with the formation of cholesteatoma. The accumulation of keratin debris is a determinant of the growth of cholesteatoma. And ear cholesteatoma is an ideal medium for bacteria. To explore the relationship between bacterial infection and cholesteatoma, we investigated in vitro the effects of Iipoteichoic acid LTA (a Gram-positive bacterial cell wall component) on keratinocyte proliferation and differentiation. Basal keratinocytes were prepared from the skin of newborn mice and cultured. Cell growth phase, adding different concentrations of LTA (0 ~ 100μg / ml). Cells were then labeled with ~ 3H-dipyridamole or ~ 3H-leucine and incubated for 24 hours. ~ 3H thymidine or ~ 3H leucine infiltrated into keratinocytes was measured with a scintillation counter. And by detecting the number of keratinocyte envelope and transglutaminase