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目的以2010年4月-2010年10月从手术室取材的43例新鲜胃癌组织标本为研究对象,研究胃癌细胞DNA的异倍体率和S期分数(SPF>15%)百分率与肿瘤临床病理因素的相关性。方法采用流式细胞仪测定43例胃癌细胞DNA含量及倍体、S期分数以及对应的癌旁细胞的DNA含量及倍体、S期分数,对照临床资料进行分析。结果胃癌组织细胞的异倍体率及SPF(>15%)的百分率与癌旁组织细胞的差别有统计学意义(P<0.01)。在肿瘤的分化程度(高中分化、低分化及印戒细胞癌,P<0.05)、浸润程度(黏膜及黏膜下、肌层、肌层至浆膜外,P<0.05)、TNM分期(Ⅰ-Ⅱ、Ш-Ⅴ期,P<0.05)之间的差别有统计学意义,而在淋巴结转移(阳性、阴性,P>0.05)差别无统计学意义。结论用流式细胞仪分析胃癌DNA含量及倍体类型、S期分数对疾病的早期诊断、研究胃癌的生物学行为,了解疾病预后、指导临床综合治疗具有重要价值。
OBJECTIVE: To study the relationship between the percentage of aneuploidy and the percentage of S phase (SPF> 15%) in gastric cancer cells and the clinicopathological features of tumor from 43 fresh gastric cancer specimens taken from the operating room from April 2010 to October 2010. Correlation of factors. Methods The DNA content, ploidy and S phase of 43 gastric cancer cells were determined by flow cytometry. The DNA contents, ploidy, S phase scores and the control clinical data were also analyzed. Results The rates of aneuploidy, the percentage of SPF (> 15%) and the number of paracancerous cells in gastric cancer tissue were statistically significant (P <0.01). (P <0.05), the degree of infiltration (mucosa and submucosa, myometrium, myometrial to serosa, P <0.05), TNM stage (Ⅰ- Ⅱ, Ⅲ-Ⅳ, P <0.05), but there was no significant difference in lymph node metastasis (positive, negative, P> 0.05). Conclusion It is of great value to analyze the biological behavior of gastric cancer, understand the prognosis of the disease and guide clinical comprehensive treatment by analyzing the content of DNA and the type of ploidy and the S phase score of gastric cancer by flow cytometry.