Effects of hydroxybenzoic acid, an important autotoxic substance in roots, on plant growth, photosynth-esis and Fusarium oxysporum occurrence in succession cropping of strawberry were evaluated in this paper. It was found that plant growth was negatively regulated by hydroxybcnzoic acid or inoculation with E oxysporum. Compared with these single factor treatments, the combination of the hydroxybenzoic acid treatment and E oxysporum inoculation caused more severe inhibition in plant growth, greatly enhanced the occurrence of disease symptoms, and significantly decreased the chlorophyll content, net photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO2 concentration. In the meantime, the chlorophyll fluorescence parameters in strawberry were also significantly affected. After the application of hydroxybenzoic acid, the original chlor-ophyll fluorescence rapidly increased, resulting in a combined corresponding decrease in the maximum chlorophyll fluorescence and the chlorophyll fluorescence transformation efficiency. The effects of hydroxybenzoic acid treatment on the above chlorophyll fluorescence parameters from inoculation were delayed. Similarly, the coordination of hydroxybenzoic acid and E oxysporum showed an elevated negative effect on the degree of inhibition of leaf photosynthesis more than the single factor treatments.