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目的:建立海南青牛胆药材中盐酸巴马汀的HPLC含量测定方法。方法:采用高效液相色谱法,Agilent TC-C18(4.6 mm×250 mm,5μm)柱,流动相为乙腈-0.4%三乙胺水溶液(24∶76),用磷酸调pH至4.0,流速1.0 mL.min-1,检测波长347 nm,柱温40℃。结果:盐酸巴马汀在线性范围0.006～0.15 mg(r=0.999 9)。平均回收率为99.8%,RSD 0.8%。结论:该测定方法精密度高,重复性好,可作为海南青牛胆中盐酸巴马汀的含量测定方法。
Objective: To establish a HPLC method for the determination of palmatine hydrochloride in Hainan bovine gallbladder. METHODS: The mobile phase consisted of acetonitrile-0.4% triethylamine (24:76) with high performance liquid chromatography on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) mL.min-1, detection wavelength of 347 nm, column temperature 40 ℃. Results: The linear range of palmatine hydrochloride was 0.006-0.15 mg (r = 0.999 9). The average recovery rate was 99.8%, RSD 0.8%. Conclusion: The method is accurate, reproducible and can be used as a method for the determination of palmatine hydrochloride in Hainan bovine gall.