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目的建立高效液相色谱测定宁神补心片中丹参酮IIA含量的方法。方法利用Lichrospher5C18色谱柱,流动相为甲醇水(73∶27);检测波长为270nm;流速为1.6mL·min-1;柱温为30℃。结果进样量在0.208～1.664μg范围内呈现良好的线性关系,回归方程为Y=2.735×107X+8.870×105,r=0.9996,平均回收率为98.54%,RSD为1.33%(n=5)。结论该方法快速简便,准确可靠,灵敏度高,可用于宁神补心片的质量控制。
Objective To establish a method for the determination of tanshinone II A in Ningshen Buxin Tablets by HPLC. Methods A Lichrospher 5C18 column was used. The mobile phase was methanol water (73∶27). The detection wavelength was 270 nm. The flow rate was 1.6 mL·min-1. The column temperature was 30°C. The results showed good linearity in the range of 0.208～1.664μg. The regression equation was Y=2.735×107X+8.870×105, r=0.9996. The average recovery was 98.54% and the RSD was 1.33% (n=5). . Conclusion The method is rapid, simple, accurate and reliable with high sensitivity and can be used for quality control of Ningshen Buxin Tablets.