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目的:通过大鼠乳腺癌癌前病变结合肝郁证造模研究,观察大鼠在癌前病变病证结合造模阶段生物学行为、激素水平、组织病理学的改变,探讨成模规律,健全造模评价指标。方法:实验大鼠随机分为空白对照组、二甲基苯蒽造模组、雌二醇造模组、二甲基苯蒽加夹尾造模组、雌二醇加夹尾造模组,采用二甲基苯蒽灌胃复制乳腺癌癌前病变大鼠模型,苯甲酸雌二醇和黄体酮肌注复制乳腺增生大鼠模型,夹尾激惹法复制肝郁证模型。观察各时期大鼠一般情况、5-羟色胺检测、组织病理学的改变。结果:①病证结合造模两组大鼠与单纯疾病造模两组大鼠比较,一般行为更符合肝郁证特点(P<0.01),5-羟色胺检测有显著差异(P<0.01);②在组织形态方面,第8周二甲基苯蒽加夹尾造模组癌前病变出现率明显高于二甲基苯蒽造模组(P<0.05),第12周二甲基苯蒽加夹尾造模组与二甲基苯蒽造模组癌前病变和肿瘤出现率无显著差异(P>0.05),但均明显高于雌二醇造模组和雌二醇加夹尾造模组(P<0.01)。结论:二甲基苯蒽灌胃加夹尾激惹能成功复制大鼠乳腺癌癌前病变肝郁证模型。该模型大鼠的一般行为、神经内分泌表现符合中医肝郁证特点。这种病证结合造模方法能缩短癌前病变动物造模时间,提高造模成功率。
OBJECTIVE: To observe the changes of biological behaviors, hormone levels and histopathology in rats with premalignant lesion of the liver and stagnation of liver-qi syndrome in rats with precancerous lesions and syndromes, Modeling evaluation index. Methods: The experimental rats were randomly divided into blank control group, dimethylbenzene anthracene modeling group, estradiol modeling group, dimethylphenylanthra plus clipping modeling group, estradiol plus clipping modeling group, Dibenzyl anthracene intragastric replication of precancerous lesions in rats with rat model, estradiol benzoate and progesterone intramuscular injection of hyperplasia rat model of replication, cat litter irritation replication model of liver depression. Observe the general situation of rats in each period, serotonin test, histopathological changes. Results: ①Compared with the model group, the general behaviors of the two groups were more consistent with the features of liver depression syndrome (P <0.01), and the serotonin detection was significantly different (P <0.01). (2) In the aspect of tissue morphology, the incidence of precancerous lesion in dimethylphenanthrene plus tailing model group was significantly higher than that of dimethylbenzene anthracene modeling group in the 8th week (P <0.05). In the 12th week, There was no significant difference in the incidence of precancerous lesions and tumor between tail model and dimethylbenzene anthracene model group (P> 0.05), but both of them were significantly higher than that of estradiol model group and estradiol plus tail model group (P <0.01). CONCLUSION: Dibenzyl anthracene plus gavage stimulation can successfully replicate the rat model of precancerous lesions of liver and liver in rats. The general behavior of the model rats, neuroendocrine performance in line with traditional Chinese medicine liver disease characteristics. This disease combined with modeling methods can reduce the pre-cancer animal modeling time, improve the success rate of modeling.