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目的观察坐骨神经分支选择性损伤(SNI)模型大鼠不同时间点术侧腰段L4~L6脊髓背角神经元磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)和磷酸化活化转录因子2(p-ATF2)的表达情况,探讨脊髓背角p-p38MAPK和p-ATF2在神经病理性痛模型不同阶段中的作用。方法健康雄性SD大鼠36只,完全随机分为空白对照组、假手术组和手术组,各12只。通过结扎腓总神经及切断胫神经,保留腓肠神经的方法建立SNI大鼠模型。观察造模前、造模后3天和14天术侧足跖缩足阈值(PWT);免疫荧光法检测造模后3天和14天术侧腰段脊髓背角p-p38MAPK和p-ATF2阳性细胞表达情况。结果选模后3天和14天,手术组大鼠术侧足跖PWT较假手术组与空白对照组明显降低(P<0.01),假手术组大鼠与空白对照组大鼠比较差异无统计学意义(P>0.05)。造模后3天和14天,手术组大鼠术侧腰段脊髓背角p-p38MAPK和p-ATF2阳性细胞表达率较假手术组和空白对照组均明显升高(P<0.01),假手术组和空白对照组SNI模型大鼠造模后各时间点,术侧腰段脊髓背角p-p38MAPK和p-ATF2阳性细胞表达率差异均无统计学意义(P>0.05)。结论 SNI模型神经病理痛的产生和维持可能与术侧腰段脊髓背角p-p38MAPK和p-ATF2表达上调有关。
Objective To observe the effects of p-p38 mitogen-activated protein kinase (p38) and phosphorylation activated transcription factor 2 (p38) on the spinal dorsal horn neurons in the lumbar spinal cord of the sciatic nerve branch selective injury (SNI) p-ATF2) expression in the spinal cord dorsal horn p-p38MAPK and p-ATF2 in neuropathic pain model at different stages of the role. Methods Thirty-six healthy male Sprague-Dawley rats were randomly divided into blank control group, sham operation group and operation group, 12 rats each. The SNI rat model was established by ligating the common peroneal nerve and cutting off the tibial nerve and preserving the sural nerve. The thresholds of PWT were observed at 3 days and 14 days after modeling. The expression of p-p38MAPK and p-ATF2 in lateral lumbar spinal dorsal horn were detected by immunofluorescence method at 3 days and 14 days after modeling. Positive cell expression. Results At 3 and 14 days after model selection, the PWT of the lateral palsy in the operation group was significantly lower than that in the sham operation group and the blank control group (P <0.01), but there was no statistical difference between the sham operation group and the blank control group Significance (P> 0.05). The expression of p-p38MAPK and p-ATF2 positive cells in the lumbar spinal dorsal horn in operation group were significantly higher than those in sham-operation group and blank control group (P <0.01) at 3 days and 14 days after operation. There was no significant difference in the expression rates of p-p38MAPK and p-ATF2 positive cells in the spinal cord of the spinal cord between the operation group and the blank control group at each time point (P> 0.05). Conclusions The occurrence and maintenance of neuropathic pain in SNI model may be related to the up-regulation of p-p38MAPK and p-ATF2 in spinal dorsal horn of operative side.