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目的:阐明黑乳海参皂苷nobilisideⅠ和nobilisideⅡ的体外抗真菌及抗肿瘤活性。方法:对黑乳海参的总提取物采取硅胶柱色谱及半制备HPLC等分离手段进行活性追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以SRB法评价化合物的抗肿瘤活性。结果:分离得到了2个新的三萜皂苷元类化合物,命名为nobilisideⅠ和nobilisideⅡ。2个新化合物均显示了较强的体外抗真菌及抗肿瘤活性。结论:通过该研究为研制新的抗肿瘤药物提供了先导化合物,为充分开发利用我国的海洋生物资源提供了物质基础和科学依据。
Objective: To elucidate the antifungal and antitumor activities of nobiliside Ⅰ and nobiliside Ⅱ of black milk ginsenosides in vitro. Methods: The total extract of black sea cucumber was subjected to silica gel column chromatography and semi-preparative HPLC separation. The chemical structure was identified by physico-chemical properties and spectroscopic methods. The anti-tumor activity of the compound was evaluated by SRB method. Results: Two new triterpene saponins were isolated and named as nobiliside I and nobiliside II. Two new compounds showed strong antifungal and anti-tumor activity in vitro. Conclusion: This study provides the lead compounds for the development of new anti-tumor drugs and provides the material basis and scientific basis for the full exploitation and utilization of marine living resources in China.