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考察了蛇毒降纤酶在不同pH缓冲液的稳定性。以酶联免疫吸附法(ELISA)测定了蛇毒降纤酶的抗原活性稳定性,并与凝固时间法测定的生物活性稳定性进行了比较。降纤酶抗原活性与其生物活性随时间降低的趋势一致(pH 3.6的缓冲液除外)。较高浓度的降纤酶在中性pH 6~7的缓冲液中比较稳定,40oC 放置10天后,仍能保持其原有活性(100BUmL-1) 的95% 以上。而较低浓度的降纤酶(5BUmL-1)水溶液相对不稳定,尤其在酸性(pH 3.6)或碱性(pH 9)条件下很容易失去活性。加入Triton X-100或牛血清白蛋白,由于降低了表面吸附,可显著提高降纤酶水溶液的稳定性(P<0.005)。
The stability of snake venom defibrase in different pH buffers was investigated. The stability of antigenic activity of snake venom defibrase was measured by enzyme-linked immunosorbent assay (ELISA), and compared with the bioactivity stability determined by the coagulation time method. Defibrinogen antigen activity is consistent with its decreasing bioactivity over time (except for pH 3.6 buffer). The higher concentration of defibrase in the neutral pH 6 ~ 7 buffer more stable, 40oC after 10 days, can still maintain its original activity (100BUmL-1) of more than 95%. The lower concentration of defibrase (5BUmL-1) aqueous solution is relatively unstable, especially in acid (pH 3.6) or alkaline (pH 9) conditions easily lose activity. The addition of Triton X-100 or bovine serum albumin significantly increased the stability of aqueous defibrase (P <0.005) due to reduced surface adsorption.