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目的观察PTD-OD-HA融合蛋白对BALB/c裸鼠皮下K562细胞实体瘤的治疗作用。方法采用皮下注射K562细胞的方法建立裸鼠皮下实体瘤模型,每两天向实体瘤中间部位多点注射200μl浓度为10 mg/ml的PTD-OD-HA融合蛋白,观察裸鼠瘤体生长情况;HE染色观察肿瘤组织病理学变化;TUNEL法和透射电镜观察肿瘤组织细胞的凋亡。结果经PTD-OD-HA治疗的肿瘤体积明显缩小;肿瘤组织切片经HE染色可见细胞核浓缩和细胞染色加深;TUNEL法和透射电镜检测可见肿瘤细胞发生了凋亡的改变。结论在BALB/c裸鼠体内,PTD-OD-HA融合蛋白具有抑制K562细胞增殖和促进其凋亡的作用。
Objective To observe the therapeutic effect of PTD-OD-HA fusion protein on solid tumor of subcutaneous K562 cells in BALB / c nude mice. Methods The model of subcutaneous solid tumor in nude mice was established by subcutaneous injection of K562 cells. Two days later, 200μl of PTD-OD-HA fusion protein at a concentration of 10 mg / ml was injected into the middle part of the solid tumor to observe the growth of the tumor The histopathological changes of the tumor were observed by HE staining. The apoptosis of tumor cells was observed by TUNEL and transmission electron microscopy. Results The volume of tumor treated with PTD-OD-HA was significantly reduced. The nuclei were concentrated and the cell staining was deepened by HE staining. TUNEL and transmission electron microscopy showed that the tumor cells were apoptotic. Conclusion The PTD-OD-HA fusion protein can inhibit the proliferation and promote the apoptosis of K562 cells in BALB / c nude mice.