用反相高效液相色谱法在ＯＤＳ柱上以甲醇－０．１ｍｏｌ／Ｌ磷酸二氢钾液（ｐＨ３．０）－０．０２ｍｏｌ／Ｌ庚烷磺酸钠液（５８．５：３５．５：６）为流动相，采用时间程序设定检测波长，选用醋酸洗必泰为内标物对定喘止咳胶囊中氨茶碱、盐酸溴己新和扑尔敏进行了分离和含量测定，方法简便，准确，各组份的线性关系良好，平均回收率：氨茶碱９９．８％，ＲＳＤ１．２７％；盐酸溴己新９９．５％，ＲＳＤ１．２９％；扑尔敏９６．４％，ＲＳＤ１．２９％（ｎ＝６）。 Reversed phase high performance liquid chromatography (HPLC) was performed on an ODS column with methanol-0.1 mol / L potassium dihydrogen phosphate solution (pH 3.0) -0.02 mol / L sodium heptanesulfonate solution : 6) as the mobile phase, using the time program to set the detection wavelength, the selection of chlorhexidine acetate as the internal standard of Dingchuan Cough Capsules aminophylline, bromhexine hydrochloride and chlorpheniramine were isolated and assayed, the method The average recovery rate was 99.8% for aminophylline, RSD 1.27%, bromhexine hydrochloride 99.5%, RSD 1.29%, chlorpheniramine 96.4% , RSD 1.29% (n = 6).