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该文将双分子标记法用于厚朴与凹叶厚朴木质素类成分的评价中,首先通过比较厚朴和凹叶厚朴化学成分,发现二者叶片中magnolignanⅠ的含量存在显著差异;然后进行厚朴与凹叶厚朴转录组测序,并选择与厚朴木质素类成分结构最相似的p-hydroxyphenyl lignin,进行生物合成关键酶基因挖掘。通过比较厚朴木质素类成分相关同源基因在厚朴和凹叶厚朴中的表达水平和结构变异,筛选获得1个编码肉桂醇脱氢酶基因片段作为厚朴木质素类成分双分子评价的候选标记,为进一步建立厚朴优劣双分子评价方法提供技术支撑。
In this paper, bimolecular marker method was applied to the evaluation of Magnolia officinalis and Magnoliaceae Magnoliaceae composition. Comparing Magnolia officinalis and Magnoliaceae Magnoliaceae chemical composition, Park and Magnoliaceae Magnoliae transcriptome sequencing, and select the composition of Magnoliaceae structural components most similar to p-hydroxyphenyl lignin, biosynthesis key enzyme gene mining. By comparing the expression levels and structural variations of Magnolia officinalis-related homologous genes in Magnolia officinalis and Magnolia officinalis, one fragment encoding the cinnamyl alcohol dehydrogenase gene was screened as a candidate for bimolecular evaluation of Magnolia officinalis components Mark, in order to further establish the pros and cons of Magnolia bimolecular evaluation methods to provide technical support.