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目的:建立HPLC法测定槐角中槐角苷含量测定方法并检测不同来源槐角中槐角苷的含量。方法:Luna 5u C18(2)100A柱(250 mm×4.60 mm,5μm),柱温为35℃,流动相为甲醇-乙腈-1%冰醋酸(40:5:55),流速为1.0 ml/min,检测波长为260 nm。结果:槐角苷质量浓度在14.58 mg/L～53.46 mg/L范围内呈良好的线性关系,回归方程为y=110353x-274717(r=0.9999),平均回收率为99.5%,RSD为1.27%;不同来源槐角中槐角苷含量范围4.27%～8.78%。结论:该方法简便、快速、灵敏、可靠;不同来源的槐角中槐角苷含量有较大差异,其中以陕北黄土高原品种含量最高,可为槐角的质量保证提供依据。
OBJECTIVE: To establish a HPLC method for the determination of acaclycidin in Huaijiaojiao and to determine the content of acaclycidin in Huaijiao from different sources. Methods: Luna 5u C18 (2) 100A column (250 mm × 4.60 mm, 5 μm) was used. The column temperature was 35 ℃ and the mobile phase was methanol-acetonitrile- min, the detection wavelength is 260 nm. Results: The results showed that there was a good linear relationship between the concentration of sophoricoside in the range of 14.58 mg / L and 53.46 mg / L, the regression equation was y = 110353x-274717 (r = 0.9999), the average recovery was 99.5% and the RSD was 1.27% ; Different sources of Sophora japonica sinensis glycoside content range 4.27% ~ 8.78%. Conclusion: The method is simple, rapid, sensitive and reliable. There are great differences in the content of acacia from different sources. Among them, the content of acacia in the Loess Plateau is the highest, which can provide the basis for the quality assurance of acacia.