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目的对大脑中线结构进行三维重建、第三脑室体积测量分析,为第三脑室前中部肿瘤切除的手术方法提供解剖学依据。方法选取10例经福尔马林固定的成年大脑标本(男、女各5例),经火棉胶包埋,分别制成厚500μm的水平位和冠状位火棉胶薄层切片,并对其进行观察、图像采集,选取其中一组女性水平位切片图像应用Amira软件进行三维重建,并对10例标本第三脑室进行体积测量。结果三维重建结果清晰显示了大脑中线结构胼胝体、穹窿、前连合、第三脑室、丘脑及丘脑间粘合的形态及毗邻关系,男性第三脑室平均体积为(1170.03±148.04)mm3,女性为(854.64±43.59)mm3,P>0.05,无统计学意义。结论对大脑中线结构的三维重建清晰的展示各个结构的立体形态和毗邻关系,为胼胝体-穹窿间手术入路切除第三脑室肿瘤提供了形态学依据,同时排除丘脑间粘合影响的第三脑室体积测量及男女体积差异性分析为脑室内镜手术操作空间提供了重要的参考价值。
Objective To reconstruct the midline structure of the brain by three-dimensional reconstruction and measure the volume of the third ventricle to provide anatomical basis for the surgical method of tumor resection in the third ventricle. Methods Ten formalin-fixed adult brain samples (5 males and 5 females) were selected and embedded in flagellin to make slices with a thickness of 500 μm and coronal flagella. The observation and image acquisition were performed. One group of female horizontal slice images were selected for 3D reconstruction with Amira software, and the volumetric measurements of the third ventricle of 10 specimens were performed. Results The results of three-dimensional reconstruction clearly showed the morphology and adjacent relationship between the midline structure of the corpus callosum, the fornix, the anterior commissure, the third ventricle, the thalamus and the thalamus. The average volume of the third ventricle in men was (1170.03 ± 148.04) mm3, (854.64 ± 43.59) mm3, P> 0.05, no statistical significance. Conclusion Three-dimensional reconstruction of the brain midline structure clearly shows the three-dimensional shape and adjacent relationship of each structure, providing a morphological basis for resection of the third ventricle tumor by corpus callosum-vault surgical approach, and excluding the third ventricle Volumetric measurement and the difference of volume between men and women provide an important reference value for the operation space of endoscopic surgery.