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在甘薯微茎尖组织培养中,材料消毒选择0.05%HgCl2溶液,时间9-10分钟,培养基琼脂浓度0.7%较为适宜。茎尖分化培养首选激素6-BA,浓度为0.25-2mg/L,或NAA0.01mg/L+6-BA0.5mg/L+GA30.1mg/L,成苗率可达85%左右。
In sweetpotato tissue culture, 0.05% HgCl2 solution was used for disinfection of materials, with a time of 9-10 minutes and a medium agar concentration of 0.7%. The optimal hormone 6-BA for shoot tip differentiation culture was 0.25-2mg / L or NAA 0.01mg / L + 6-BA 0.5mg / L + GA 30.1mg / L.