应用ＨＰＬＣ法改进血清腺苷脱氨酶（ＡＤ）活性的测定，对该酶反应主要因素如血清浓度、底物特异性进行比较实验。并测定３４例健康人血清ＡＤ活性，其正常活性值０．２５５±０．０７５，此法较传统测氨法具有较多优点，如准确性高、重复性好和操作简便。此外，还可适用于检测血清中尿酸含量变化，有助于诊断肾脏疾病和老年痛风症。 HPLC method was used to improve the activity of serum adenosine deaminase (AD), and the main factors of the enzyme reaction such as serum concentration and substrate specificity were compared. The activity of AD in serum of 34 healthy people was measured, whose normal activity value was 0.255 ± 0.075. This method has many advantages over the traditional method such as high accuracy, good repeatability and easy operation. In addition, it can be applied to detect changes of serum uric acid, which can help to diagnose kidney disease and elderly gout.