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目的探讨幽门螺杆菌(Hp)感染与胃癌中信号通路中效应蛋白(Shh)和反式激活活性蛋白(C-myc)表达的关系,可作为胃上皮组织发生肿瘤化的重要指示基因。方法采用免疫组化法对100例胃癌组织及46例正常胃上皮组织中的Shh及C-myc进行检测,然后采用快速尿素酶试验及组织病理学检测两种临床检测方法对Hp进行检测,以了解Shh与C-myc在胃癌组织中的表达及其与Hp感染之间的关系。结果胃癌组织中Shh的平均染色得分为(4.02±2.31)分,明显高于正常胃上皮的染色总得分(1.19±0.87)分(P<0.05);胃癌组织中C-myc的平均染色得分为(4.59±2.58)分,明显高于正常胃上皮的染色总得分(2.11±1.77)分(P<0.05);Hp感染呈阳性与阴性胃癌中,Shh表达的阳性率分别为23.68%与69.35%,差异有统计学意义(P<0.05);在Hp感染呈阳性与阴性胃癌中,C-myc表达的阳性率分别为54.76%、74.14%,差异无统计学意义。结论胃癌组织中的Shh及C-myc两种基因的表达与Hp感染存在着一定的关系,可作为胃上皮组织发生肿瘤化的重要指示基因。
Objective To investigate the relationship between Helicobacter pylori (Hp) infection and the expression of Shh and C-myc in gastric cancer, which may serve as an important indicator of tumorigenesis in gastric epithelial tissues. Methods Immunohistochemical method was used to detect Shh and C-myc in 100 cases of gastric carcinoma and 46 cases of normal gastric epithelium. Hp was detected by rapid urease test and histopathological examination. To understand the expression of Shh and C-myc in gastric carcinoma and its relationship with Hp infection. Results The average staining score of Shh in gastric cancer was (4.02 ± 2.31) points, which was significantly higher than that of normal gastric epithelium (1.19 ± 0.87) (P <0.05). The average staining score of C-myc in gastric cancer was (4.59 ± 2.58), which was significantly higher than that of normal gastric epithelium (2.11 ± 1.77) (P <0.05). The positive rates of Shh expression in Hp positive and negative gastric cancer were 23.68% and 69.35% (P <0.05). The positive rates of C-myc expression in Hp-positive and -negative gastric carcinomas were 54.76% and 74.14%, respectively, with no significant difference. Conclusion The expression of Shh and C-myc in gastric cancer has some relationship with Hp infection, which may be used as an important indicator of tumorigenesis in gastric epithelial tissue.