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The protective role of a human erythro-cyte-derived depressing factor (EDDF) on blood vessels was evaluated. The experiments were carried out on 25 male Wistar rats aged 6-8 weeks, which were divided into control (n=8), calcium overload (n=8) and NG-L-nitro-arginine hypertensive model groups (L-NNA, n = 9), respectively. The isolated vascular ring perfusion assay, two-photon laser scanning fluorescence microscopy (TPM) and transmitted electron microscope were used to examine the effect of EDDF on vascular function and ultrastructure. Results showed that the contractile response of calcium overload rats and L-NNA rats to phenylephrine (PE) was significantly enhanced compared with that of the control (P < 0.05), and EDDF (10-3g·mL-1) remarkably decreased the vascular contractile response of control’s and calcium overload rats (P < 0.05), while EDDF had no effect on that of L-NNA rats. EDDF also alleviated the ultrastructural lesion of aorta VSMC in calcium overload rats by easing the abnormal in the nucle
The protective role of a human erythro-cyte-derived depressing factor (EDDF) on blood vessels was evaluated. The experiments were carried out on 25 male Wistar rats aged 6-8 weeks, which were divided into control (n = 8), calcium The isolated vascular ring perfusion assay, two-photon laser scanning fluorescence microscopy (TPM) and transmitted electron microscope (N = 8) and NG-L-nitro-arginine hypertensive model groups were used to examine the effect of EDDF on vascular function and ultrastructure. Results showed the contractile response of calcium overload rats and L-NNA rats to phenylephrine (PE) was significantly enhanced compared with that of the control (P <0.05), and EDDF (10-3g · mL-1) remarkably decreased the vascular contractile response of control of and calcium overload rats (P <0.05), while EDDF had no effect on that of L-NNA rats. EDDF also alleviated the ultrastructural lesion of aorta VSMC in calcium overload rats by easing the ab normal in the nucle