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RNA干涉(RNA interference,RNA i)是生物体内通过双链RNA(doub le-stranded RNA,dsR-NA)抵抗病毒入侵和抑制转座子活动的一种自然机制。21~25nt大小的小干涉RNA(small interfering RNA,siRNA)作为RNA i过程中关键效应分子,在哺乳动物细胞能够高特异性、高效性地抑制基因的表达,因而在研究基因功能、表达调控、信号传导通路和基因药物开发方面显示其显著的优越性。这项技术也被应用于泌尿系统肿瘤的研究,主要针对泌尿系统肿瘤的发生、发展机制及治疗等方面进行探讨。本文就siRNA在泌尿系统肿瘤领域内的应用研究作一综述。
RNA interference (RNAi) is a natural mechanism in vivo to resist virus invasion and inhibit transposon activity through double-stranded RNA (dsR-NA). As a key effector of RNAi, small interfering RNA (siRNA) of 21-25 nt in size can inhibit gene expression in mammalian cells with high specificity and high efficiency. Therefore, the study of gene function, expression regulation, Signaling pathways and gene drug development has shown its significant superiority. This technique has also been applied to the study of urological tumors, mainly for the occurrence of urological tumors, mechanisms of development and treatment. This article reviews the application of siRNA in the field of urinary system tumors.