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目的:观察中药脑疏宁对创伤性脑损伤大鼠基质金属蛋白酶基质金属蛋白酶(MMPs)表达的影响。方法:采用免疫组化方法检测脑组织MMPs蛋白的表达,测定脑组织含水量及伊文思蓝(EB)含量,电镜下观察血脑屏障超微结构改变。结果:创伤组大鼠伤侧脑组织含水量、伊文思蓝(EB)含量及伤灶周围基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)的表达水平均明显高于假手术组(P<0.01);血脑屏障结构破坏。脑疏宁组脑组织含水量、EB含量及基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)的表达水平均明显低于创伤组(P<0.05,P<0.01);血脑屏障结构较为完整。结论:脑疏宁可通过改善血脑屏障损伤减轻创伤性脑水肿,其保护血脑屏障的作用可能是通过抑制MMP-2和MMP-9蛋白表达实现的。
OBJECTIVE: To observe the effect of Chinese herbal medicine Naoshuning on the expression of matrix metalloproteinases and matrix metalloproteinases (MMPs) in rats with traumatic brain injury. METHODS: The expression of MMPs protein in brain tissue was detected by immunohistochemical method. The water content and Evans blue (EB) content in brain tissue were measured. Ultrastructural changes of blood-brain barrier were observed under electron microscope. Results: The expression of MMP-2, MMP-2 and matrix metalloproteinase-9 (MMP-9) in the injured brain tissue of the traumatic brain tissue were significantly different. It was higher than the sham group (P<0.01); the blood-brain barrier structure was destroyed. The brain water content, EB content, expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) were significantly lower in Naoshuning group than in trauma group (P<0.05, P<0.01). ); blood-brain barrier structure is more complete. Conclusion: Naoshuning can reduce traumatic brain edema by improving blood-brain barrier injury. Its protective effect on blood-brain barrier may be achieved by inhibiting the expression of MMP-2 and MMP-9 protein.